- In the DC Universe, Ace is the name of several villains, all of whom were members of the Royal Flush Gang.
- In the Wild Cards book/short story series, Aces are the classification given to people who have developed super powers.
- Ace is part of a superhero duo in the recurring Saturday Night Live sketch The Ambiguously Gay Duo.
Characters with Ace as part of their name
There are also a number of characters who have Ace as part of their name.